Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
Notes
Laboratory-performance Study of the Notified Methods to Detect Genetically Modified Papaya (55-1)
Hiroyuki KIKUCHITakahiro WATANABEKikuko KASAMAChiseko WAKUIAkihiko MATSUKIHiroshi AKIYAMATamio MAITANI
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2005 Volume 46 Issue 1 Pages 21-27

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Abstract

To investigate important factors affecting the reliability of the analytical results, proficiency tests were attempted for the histochemical method (GUS method) and the qualitative PCR method (PCR method) to detect genetically modified papaya (55-1) in the Japanease official method. The test samples were distributed to twenty-three laboratories that participated in the study and were examined according to the protocol. All the data collected from participating laboratories were statistically analyzed. In the PCR method, one negative sample was detected as positive using detection primers in one laboratory, though the sample was negative when checked using confirmation primers. Contamination might have occurred in the step of the preparation of the PCR sample solution using detection primers. In the GUS method, all the test samples were identified as expected. Thus, all the laboratories reported correct results overall.

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© 2005 Japanese Society for Food Hygiene and Safety
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