Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
Validation Study
Validation Study of Analytical Method for Determination of Amnesic Shellfish Poison in Bivalves
Akemi YAMAMOTO Shiho KUDOMinoru NAKAYARyuji HANAISHIYukiyasu MASUDAJunko KIMURAAsae SAKURABAMegumi SHIBATASho KUDOTakeo ITSUKAICHIHirohisa SATOAtsuko MURAKAMIAkiko KOGAWA
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2017 Volume 58 Issue 6 Pages 281-287

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Abstract

Amnesic shellfish poison (ASP) is regarded as one of the shellfish poison groups in the EU, though it is not subject to regulation in Japan. We have developed an analytical method of ASP based on the report by Hatfield et al. and other methods. Validation studies were carried out with certified compositional reference materials (CRM). Performance parameters were estimated based on 17 analytical results. The estimate of trueness was 97.5%, and the estimate of intralaboratory reproducibility (RSD) was 1.5%. The HorRat(r) value was 0.16. These performance parameters meet the criteria in the Codex Procedural Manual. Furthermore, internal quality control was performed by using the CRM. The action limits were set based on the performance parameters of the method. Most of the results of the internal quality control were within the action limit range. The results confirmed that the quality of the analyses was well maintained. The purpose of the analytical method is to confirm that the level of ASP in scallop is less than 4.6 mg/kg. The applicability of the analytical method to scallops was confirmed by using spiked samples.

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© 2017 Japanese Society for Food Hygiene and Safety
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