Attempt to Calculate the Efficiency of Methylation Using ¹H-NMR for Analyzing Brominated Vegetable Oil

Abstract

We studied the efficiency of methylation for analyzing brominated vegetable oil (BVO). In this report, we investigated whether ¹H-NMR is an applicable method for assessing the efficiency of methylation to analyze BVO. ¹H-NMR sufficiently calculated the efficiency of methylation using each integral and the numbers of protons derived from the methyl group, which is characteristic in products, and the methine group, which is characteristic in unreacted substances. Additionally, the efficiency of methylation calculated via ¹H-NMR was in good agreement with changes in the peak area of BVO fatty acid methyl esters (BVOFAMEs) after various heating times obtained from GC-FID analysis. Therefore, ¹H-NMR is applicable for calculating the efficiency of methylation to analyze BVO.