Studies on Soft Drinks (VII)

Part II Preservatives in Soft Drinks (6) Acute Toxicity and Moldstatic Activity for Aspergillus niger of Dehydroacetic Acid and its Transformation Products

Abstract

Acute toxicity and moldstatic activity for Asp. niger of transformation products of dehydroacetic acid were investigated.
And the results obtained were as follows:
1) The moldstatic activity of each compounds compared with dehydroacetic acid were found to be 1/15 of dehydroacetic acid in strength for diacetylacetone, 1/30 for 2, 6-dimethyl-4-pyrone-3-carboxylic acid, and 1/180 for 2, 6-dimethyl-4-pyrone.
2) On acute toxicity, the oral LD₅₀ (and 95% confidence limits) in mice were found to be 1.33g/kg (1.23-1.44g/kg) for dehydroacetic acid, 2.33g/kg (2.04-2.65g/kg) for 2, 6-dimethyl-4-pyrone-3-carboxylic acid, 1.67g/kg (1.49-1.88g/kg) for 2, 6-dimethyl-4-pyrone, and 0.65g/kg (0.59-0.72g/kg) for diacetylacetone.