ブタの体内残留抗生物質の検出とディスク法の応用について
1973 年 14 巻 4 号 p. 384-386
詳細
1973 年 14 巻 4 号 p. 384-386
Detection of residual antibiotics, such as penicillin (PC), streptomycin (SM), and oxytetracyclin (OTC) in slaughtered swine carcases was made by the standard bioassay technique, using Sarcina lutea ATCC 9341, Bacillus subtilis ATCC 6633, and Bacillus cereus var. mycoides ATCC 11778, respectively.
Specimens obtained for the examination were neck muscle, liver and kidney of 29 pigs which had been treated with the above mentioned antibiotics. In addition to this field survey, an experimental study on the reliability of disk method, for the detection of penicillin incorporated in pork extract, was also carried out comparing with the standard cup method.
As the results it was found that 1 (3.7%) out of 27 muscles, 6 (29.0%) of 21 livers, and 4 (20.0%) kidney contained 0.16-1.1IU/g of PC, while 3 of 21 (14.2%) liver and 2 of 21 (9.5%) kidney contained 0.15-0.75μg/g of SM. However, none of the specimen tested contained any detectable amount of OTC.
As for the reliability of the disk method, it was found that almost the same sensitivity with that of the cup method could be achieved, especially at a lower concentration of the antibiotics. Therefore, it is recommeded to use disk method as a routine screenig procedure for the detection of residual penicillin in animal tissues.
