Abstract
The rate of N-nitrososarcosine formation from creatine and sodium nitrite was studied under the condition of pH 3.0 and 37°C, similar to that in the human stomach after ingestion of foods.
The initial rate of N-nitrososarcosine formation from creatine and sodium nitrite was calculatedd from the absorbance at 260nm after removing the interfering materials by extraction and thin-layer chromatography. It was proportional to the creatine and nitrite concentration, not to the square of the nitrite concentration under the experimental conditions; rate=k×[creatine]×[NaNO2]. The k value of the reaction from the equation was 0.66×10-3 (mol-1·l·min-1). When [amine] was 20mM and [NaNO2] was 300mM, the amount of N-nitrososarcosine formed from creatine and sodium nitrite was 4% of that of nitrosopiperidine and 0.008% of that of nitrosomorpholine.
