Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
Online ISSN : 1882-1006
Print ISSN : 0015-6426
ISSN-L : 0015-6426
Studies on Chemical Analysis of Mycotoxin (VII)
Study on Determination of Aflatoxins by High Performance Liquid Chromatography
Etsuko ISOHATAYuiko TAKEDAMitsuru UCHIYAMA
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1977 Volume 18 Issue 1 Pages 48-52_1

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Abstract

High-performance liquid chromatography of Aflatoins was carried out by using two kinds of column packings.
Silicagel (Iatrobeads 6RS-8010) yielded satisfactory separation of Aflatoxin B1, B2, G1, G2, by eluting with dichloromethane-chloroform-methanol (60:40:0.15) or chloroform-iso-octane-iso-propanol (75:25:0.5), whereas porous polymergel (Iatrobeads 6CP-2010) could be applied for the clear separation of B-group and G-group aflatoxins from patulin and penicillic acid.
The detection of aflatoxins was performed with UV-254nm or UV-360nm, while the latter is more recommendable to avoid the disturbance with coexisting materials.

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