Deoxynivalenol and Its Acetate as Feed Refusal Principles in Rice Cultures of Fusarium roseum No. 117 (ATCC 28114)

Studies on the Toxic Substances in the Infected Cereals (VI)

Abstract

An attempts was made to elucidate the substances formed in rice cultures of a trichothecene-producing strain of Fusarium roseum No. 117 (ATCC 28114) that caused feed refusal to rats. After 15 days of incubation at 25°C, concentrations of deoxynivalenol (3α, 7α, 15-trihydroxy-12, 13-epoxytrichothec-9-en-8-one, DON) and 3-acetyldeoxynivalenol (3α-acetoxy-7α, 15-dihydroxy-12, 13-epoxytrichothec-9-en-8-one, 3-Ac-DON) reached to about 220μg and about 160μg per g of rice, respectively. Additionally, a trace amount of a new trichothecene, 3α, 15-diacetoxy-7α-hydroxy-12, 13-epoxytrichothec-9-en-8-one was also isolated.
The greater part of the feed-refusal activity of the inoculated rice seems to be in the fraction containing above trichothecenes, but participation of other substances was not ruled out. Comparing two-day feed consumption as % of control, medium effective doses of pure DON and 3-Ac-DON were about 100μg and about 150μg per g of feed, respectively. Gain in the body weight of rat was completely inhibited with diets containing 150μg of DON or 200μg of 3-Ac-DON per g, receiving about 4mg of DON or about 7mg of 3-Ac-DON per kg of body weight.