Studies on Residues of Synthetic Antibacterials in Livestock Products (I)

Analytical Method of 3, 5-Dinitro-o-toluamide (Zoalene) by Gas Chromatography

Abstract

Extraction technique and determination method of 3, 5-dinitro-o-toluamide (zoalene) were described in this paper.
The mixture of sample and anhydrous sodium sulfate was ground to fine powder by a homogenizer. The mixture was packed in a glass column of 38mm in diameter tightly.
As eluting agent acetonitrile was used. Zoalene was eluted efficiently by the column chromatography, and after clean up it was treated by florisil column chromatography, condensate of eluent was made to react with heptafluoro-n-butyric anhydride. The determination was performed by ECD gas chromatography on the solution of reaction product. A linear calibration curve was obtained in range of 0.1μg/ml to 1.0μg/ml of zoalene. The lower limit of the sample size of zoalene for identification was 0.03ng.