Abstract
Spore counting media were compared for effect on the recovery of non-heat-treated and heat-treated spores of Clostridium botulinum and C. sporogenes. The spore count obtained with each of eight kinds of media was compared with that recovered with pork pea infusion agar (PPI). No difference was observed among the media in the recovery count of non-heat-treated spores. With heat-treated spores, however, the spore count recovered with yeast extract agar was largest, and was comparable to that recovered with PPI.
As regards reducing agent added to the recovery medium, no significant difference was observed between sodium thioglycollate and L-cysteine monohydrochloride. No inhibitory effect was observed when the reducing agent was added to the medium before autoclaving.
