Effects of Oral Administration of Azo Dyes on RNA Synthesis in Rat Liver Nuclei

Effects of Tar Dyes on RNA Synthesis (Part 4)

Abstract

Incorporation of ³H-UTP into RNA, and protein and RNA contents were measured in nuclear preparations from rats after two doses of Ponceau 3R, Amaranth, or Sunset Yellow FCF (300mg/kg of body weight). The incorporation of UTP was greater in nuclei from both Ponceau 3R- and Amaranth-treated rats than in those from control rats. Furthermore, two doses of Ponceau 3R increased the RNA content in liver nuclei. No significant difference was observed in the protein content of nuclei isolated from rats treated with these azo dyes or not treated. Nuclear preparations from Sunset Yellow FCF-treated rats showed no difference from the control.
These results suggest that Ponceau 3R and Amaranth stimulate in vivo RNA synthesis in rat liver nuclei.