Abstract
A droplet counter-current chromatography (DCC) method for the separation of contaminants from lipids in fish samples has been developed for food contamination monitoring by GC/MS. Concurrently, the lipid constitution of fish extract has been investigated by HPTLC-densitometry.
The main component of crude fish extract was triglyceride (TG) and the other components were diglyceride (DG), cholesterol (Ch), free fatty acid (FFA), monoglyceride (MG), phospholipids (PL), and unknowns.
Various industrial chemicals and pesticides were eluted with good separation and reproducibility from DCC according to their partition coefficients between the stationary phase and mobile phase.
After removal of almost all TG by acetonitrile n-hexane partition, the remaining lipid was subjected to DCC. Fish extract was fractionated into 8 fractions. These fractions could be concentrated to 0.1-1mL (from 1kg of fish).
