Detection and Determination of Nitrofurazone and nitrofurylacrylamide in Milk

Abstract

The methods for the detection and determination of nitrofurazone and nitrofurylacrylamide added in milk are described.
These substances, extracted by dimethylformamide, were detected by color reaction with 1N sodium hydroxyde solution. The method for the determination of nitrofurazone was based on the formation of 5-nitro-2-furfural phenylhydrazone, which was estimated by colorimetric method, and nitrofurylacrylamide was determined by the absorbance measurement of that extracted with dimethylformamide.