1962 年 3 巻 3 号 p. 292-296
[Correction] J. Food Hyg. Soc. Jpn Vol.4 No.1 (1963) pp.68o-68o
[Correction] J. Food Hyg. Soc. Jpn Vol.4 No.1 (1963) pp.68q-68q
[Correction] J. Food Hyg. Soc. Jpn Vol.4 No.1 (1963) pp.68p-68p
The methods for determination of nitrofurazone and nitrofurylacrylamide by polarography and column chromatography are described.
When nitrofurazone and nitrofurylacrylamide in N, N-dimethylformamide are deter-mined by polarography, ammonium citrate solution (pH 5.0) is an excellent buffer. Relation between their wave heights and the concentrations shows the linearity in a standard graphic paper.
However in a case of the mixture of nitrofurazone and nitrofurylacrylamide, each substance is not determined by the polarographic method. In such a case, these substances can be separated by column chromatography. Silica gel in used as an absorbent. Nitrofurylacrylamide is eluted with a mixture of ether and acetone, and nitrofurazone with acetone.
These methods have been successfully applied to the analysis of nitrofurazone and nitrofurylacrylamide in preparations and food.
