1997 Volume 38 Issue 6 Pages 412-417_1
Cardiac glycosides in “Moroheiya” (C. olitorius) and its products were analyzed as their aglycone, strophanthidin (SP), by reverse-phase HPLC with UV detection. Methanol extracts of “Moroheiya” samples were hydrolyzed with 1mol/L HCl and cleaned up with a Bond Elut Silica cartridge. Well-matured seed of C. olitorius contained large amounts of cardiac glycosides in the range of 4.1-6.6mg/g (average 5.43mg/g) as SP. The pod also contained a small amount of cardiac glycosides (0.24μg/g as SP). However, no cardiac glycosides were detected in the fresh “Moroheiya” (including green leaf, green stem, shoot and pod), which has been used in Japan as a vegetable, in dried health tea products and in health food products. Spectroscopic data suggested that there were at least two cardiac glycosides in C. olitorius seed.