デンプングリコール酸ナトリウムの生化学的分解に関する研究 (第3報)
唾液アミラーゼによる加水分解物中の低分子物質について
1968 年 9 巻 4 号 p. 281-284
詳細
1968 年 9 巻 4 号 p. 281-284
Low molecular substances produced by the hydrolysis of sodium carboxymethylstarch (Na-CMS, degree of substitution: 0.38) with saliva amylase were studied.
Na-CMS was hydrolyzed with human saliva amylase, and the hydrolyzates were fractionated by Sephadex column to No. 1-5 portions. The fraction 1 showed a different behavior from the other fractions on paper chromatogram, and it was colored to violet (absorption maximum: 540mμ) by heating with 0.01% 2, 7-dihydroxy-naphthalene sulfuric acid solution in water bath. Infrared spectrum of the fraction showed the absorption of carboxyl group. These facts revealed the presence of a carboxymethyl group in the fraction 1, but in the fraction 2-5, respectively carboxymethyl group was not identified. The fraction 1 was not colored with iodine, and the average polymerization degree of the fraction 1 was 7.5 by the determination with 3, 5-dinitrosalicylic acid method.
