日本口腔科学会雑誌
Online ISSN : 2185-0461
Print ISSN : 0029-0297
ISSN-L : 0029-0297
歯肉の炭水化物に関する組織化学的研究
I. 過沃度酸Schiff反應
森 昌彦
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ジャーナル フリー

1956 年 5 巻 1 号 p. 14-23

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I obtained a most excellent result with the periodic acid Schiff reaction for the determination of the polysaccharides in human gingiva. Better results were obtained with periodic acid sodium or kalium for oxidization than with periodic acid, Weigert's haematoxylin being perferable for nuclear staining, and Orang G for a counterstain. Carnoy and absolute alcohol is best for tissue fixation, but buffered formalin is also usable.
The periodic acid Schiff reaction shows the distribution of the polysaccharides to various degrees through out the gingiva epithelial cells, and the stainabily can be made to diminish or disappear, by the Amylase digests technic.
Also there is a close relationship between various degrees of inflammation of the gingiva, and the polysaccharides of the epitheral cells.
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