2022 Volume 88 Issue 6 Pages 494-502
The effects of dietary yuzu peel powder (YP) on growth and lipid oxidation of Epinephelus akaara were examined. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of methanol-dimethyl sulfoxide extract from YP was measured and the antioxidant compounds in the extract were identified by mass spectrometry. E. akaara was reared for 4 weeks and fed diets with and without 3% YP (test and control). Thereafter, the plasma biochemical markers and thiobarbituric acid reactive substance (TBARS) levels in the liver and meat homogenates were measured. The antioxidant compounds identified in YP included narirutin, naringin, hesperidin, and neohesperidin; hesperidin contributed the most to YP antioxidant activity. The diet containing YP had no effect on the diet amount and fish weight; however, TBARS levels in the plasma and liver homogenates were lower than those in the control group. No significant difference was observed in the TBARS levels of fresh meat between the test and control groups, whereas the TBARS level in the meat of the test group stored at 4℃ for 24 h was substantially lower than that in the control group. These results indicate that a diet containing YP does not affect the growth of E. akaara but suppresses lipid oxidation of fish meat during storage.
