NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Originals
Screening method for tetrodotoxin using immunochromatographic test kit
RYOMA MINAMIKAORI TANIGUCHIHIRONOBU MATSUOKAZUNARI TSUJIMURARYOMA MIURAYUSUKE SHIBAHARAKENTARO KAWATSUMARI YOTSU-YAMASHITATOMOHIRO TAKATANIOSAMU ARAKAWA
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2022 Volume 88 Issue 6 Pages 515-522

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Abstract

 The validity of an immunochromatographic test kit for tetrodotoxin (TTX) was examined. In the tests using the TTX certified standard, a strong negative correlation (r=−0.9658) was observed between the logarithm of TTX concentration and the intensity ratio of C- and T-lines (T/C) formed on the test plate in the final TTX concentration range of 0.0125-0.2 mouse unit (MU)/mL. When the intensity of the T-line was visually determined on a 5-level scale (++ > + > w+ > vw+ > −), with ++ and +(T/C > 0.4) as TTX negative and w+, vw+, and −(T/C < 0.4) as TTX positive, the presence/absence of TTX could be judged with a final concentration of ~0.06 MU/mL as the detection limit. Matrix effects were examined using non-toxic cultured pufferfish tissues, indicating that ultrafiltration and/or 5-fold dilution can reduce the matrix-induced decrease in T/C to a low level. A comparison of liquid chromatography-tandem mass spectrometry and TTX detection kit results using the toxic wild pufferfish livers showed that the kit produced false positives by about 20%, but no false negatives were observed at all. In conclusion, this TTX test kit is useful as a primary screening method for TTX.

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© 2022 by The Japanese Society of Fisheries Science
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