Abstract
It was found, as reported in the pr vious paper, that the gradations in the reduction of TTC by filament reflected sharply the intact, injured and dead states.
Limits of viability of filaments under various deleterious conditions were determined by using TTC as indicator.
The results are summarized as fo1lows:
1) Injurious effect of 10-4M. copper sulfate in sea water becomes manifest within 1 day, 10-6M. after 3 days, and 10-8M. after 5 days;
2) The critical temperature of causing damage after 24 hours of incubation is found at 30° to 32°C.;
3) Injury becomes apparent after 3 hours of desiccation at 66% relative humidity, and within 1 to 2 hours at 10%, respectively;
4) By the ultraviolet irradiation (quartz mercury sterilizing lamp), severe injury occurs after 30 minutes, and complete killing after an hour.
Further research in extended application of the TTC test method to other useful algae is in progress.
