抄録
The pH values of eggs, milt and coelomic fluid of mature rainbow trout were measured with Beckmann pH meter G type and their results were compared with the percentages of eyed eggs obtained by each brood fish. The results obtained are as follows:
1) The pH value of the testis has a tendency to gradually increase its value from the anterior part toward the posterior (Table 1).
2) The pH value of milt varies from 7.84 to 8.14, and averages 8.03 ± 0.05. The pH value of the transparent fluid which is stripped with milt is 6.25 in average, and this fluid coagulates the sperm and stops its movement. Therefore, care should be taken to prevent mixing this fluid into the normal sperm.
3) The sperm moves within the scope of nearly 4.5 to 10.4 in the pH value and its movement tends to be more active in basic range of pH.
4) The pH value of the egg surface is 8.10-8.59, average 8.27 ± 0.80. The pH value of egg content is 6.53-6.68, average 6.63 ± 0.01 (Table 2). The eggs which showed survival rate below 80% appeared only in the pH range below 8.2 for the egg surface (Fig. 2).
5) The pH value of the coelomic fluid is 7.61-8.14 and its value decreases as the number of broken egg in the coelomic, fluid increases (Fig. 1).
