1. It was found that there are two Hb's (component F and S) in blood of chum salmon, the ratio of these Hb's in amount being almost constant (component F 55 to component S 45).
2. Component F and S were separated from each other by means of starch electrophoresis and some properties were compared with each other. Component S is less anionic electrophoretically, more soluble in phosphate buffer, more resistant to heat coagulation, and moved slower in column chromatography than component F. The near ultraviolet spectra of component F shift towards longer wavelengths than those of component S.