Abstract
The purification of the proteinase of tunny pyloric caeca was carried out as follows; the distilled water extraction of ground material, salting-out with ammonium sulphate, dialysis, rivanol treatment for removal of amylase, fuller's earth treatment, and precipitation and cry-stallization with acetone (Fig. 3).
The crystals of proteinase were obtained in the form of needles (Fig. 4).
The crystalline proteinase was obtained with an yield of 1.5% in activity and a specific activity increased 20.6 times as compared with the original solution (Table 3).
