カツオ幽門垂中の未知因子に関する研究-I

CFと置きかえ得る未知因子の発見

抄録

Amount of CF, citrovorum factor, in pyloric coeca of skipjack was determined by bioassay with Leuconostoc citrovorum as 5.9γ per one gram of this tissue. However, the presence of a substance which favors the growth of this bacteria but behaves at variance with the known CF was also disclosed as mentioned below, suggesting the share of this unfamiliar substance, “unknown factor” so to speak, in the above value of total CF.
In the incubation of pyloric coeca of skipjack or aqueous extracts thereof, maximum liberations of citrovorum factor, including the unknown factor, took place when conjugase obtained from chick pancreas was employed or when this tissue was subjected to autolysis at pH 4.5, while the conjugase from hog kidney and also takadiastase were found less effective, simultaneous use of cysteine or ascorbic acid known as activator of conjugase having no effect on the liberation of CF from conjugated state (see Table 1).
The unknown factor was proved by the bioautographic method to be completely separable from the known CF (see Fig. 1).
As regards the promotive effect on the growth of Leuc. citrovorum, while the known CF is readily damaged when acidified to pH 2.0, the unknown factor factor can survive such acidification (See Figs. 2 and 3).