日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
マボヤ, Cynthia roretzi V. DRASCHEの生化学的研究-VI
被嚢中の擬ケラチンについて
土屋 靖彦鈴木 芳夫
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ジャーナル フリー

1962 年 28 巻 2 号 p. 222-226

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The defatted test of Cynthia roretzi v. DRASCHE contained 42.5 per cent of crude protein (Table 1). When the test was autoclaved with water at 20 pounds for 2 hours, about one-fourth of its total nitrogen dissolved in water (Table 2).
In the enzymic digestion of test, about 40 per cent of the total nitrogen was hydrolyzed by pepsin or papain but only 1.1 per cent by trypsin. On the contrary the denatured test formed by autoclaving was remarkably digested at the level of 67.7 per cent by the last enzyme (Table 3).
The amino acid composition of the scleroprotein was similar to that of ichthulokeratin and neurokeratin (Table 4). It showed low contents of cystine and serine as compared with eukeratin, and differed sharply from elastin and collagen rich in glycine, alanine and valine and poor in cystine and tryptophan. The ratio of histidine: lysine: arginine in this scleroprotein was 1:2.9:4.2, which agrees with that of ichthulokeratin and differs from 1:4:12 of eukeratin.
These results led the authors to classify this protein as a pseudokeratin.

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