Some investigations of the volatile fatty acids and the volatile bases in Shiokara from commercial source were carried out by gas-chromatographic analysis.
In the acidic fraction of Ika-Shiokara, six sorts of volatile fatty acids were found. They were identical to formic acid, acetic acid, propionic acid, iso-butyric acid, n-butyric acid and n-caproic acid. Ten sorts of base were contained in the basic fraction, six of them were identified as trimethylamine, ammonia, dimethylamine, monomethylamine, iso-butylamine and n-butylamine, however, the other components were left un-identified.
In Katsuo-Shiokara, the same components were found except iso-valeric acid and small amount of three unknown substances. In both Shiokara the chief acidic and basic ingredients were acetic acid, iso-butyric acid, propionic acid, ammonia, iso-butylamine and an another un-identified substance.