Grammistins of Grammistes sexlineatus, Diploprion bifasciatum and Aulacocephalus temmincki were fractionated by Sephadex LH-20 column chromatography and countercurrent distribution, and compared with those obtained from Pogonoperca punctata. Three grammistin components corresponding to grammistins A, B and C of P. punctata were separated from G. sexlineatus, suggesting a close relationship between these two species. Grammistins of D. bifasciatum and A. temmincki revealed a very different pattern of elution and distribution from those of P. punctata. The major grammistin from A. temmincki was a fairly large molecule. In addition to the four grammistins, D. bifasciatum contained a large amount of a fat soluble hemolysin, which was a small tertiary or quaternary amine without a peptide moiety.