Volume 41 (1975) Issue 11 Pages 1185-1189
The absorption and metabolism of dietary cholesterol-14C at various stages of the molting cycle in the prawn, Penaeus japonicus BATE, were studied.
When the prawn was fed on the diet containing cholesterol-14C, radioactive compounds were absorbed from the digestive tract and widely distributed in all tissues and organs examined. High radioactivity was detected in the hepatopancreas and hypodermis of prawn. The concentrations of radioactive compounds were high in all tissues at stages C1-C2, C3-C4, and D1-D4 of the molting cycle, but low at stages B and D3-D4. Thin-layer chromatographic analysis of lipids demonstrated a high free sterol fraction (63-98%) and a low esterified sterol fraction (0-16%) in all stages of the molting cycle. Relatively high radioactivity was also found in the polar steroid fraction at stage B.