Abstract
Upon the preparation of lymphocytes from fishes, peripheral lymphocytes were effectively separated from the blood of rainbow trout and goldfish by the following density gradient centri-fugation system: the most effectual density gradient was a stratified 12%-15%-18%-20%(w/v) Ficoll-MEM system when the blood samples were centrifuged at 100×g for 40min at 15°C. Lympho-cytes accumulated exclusively in the 18% Ficoll layer, whereas erythrocytes and other leukocytes distributed to the 20% Ficoll layer in this Ficoll density gradient centrifugation. Lymphocytes obtained from the 18% Ficoll layer demonstrated a typically morphological characters of small lymphocyte. The yield rate and viability of lymphocyte from the peripheral blood of rainbow trout were 70-80% and 98%, respectively.
