A procedure was proposed to eliminate water-soluble alginate from the crude preparation of fucose-containing Polysaccharide of brown alga Kjellmaniella crassifolia.
As reported perviously, the fucose-cintaining polysaccharide was extract from algal fronds. 1% solution of this polysaccharide was carefully precipitated with 20% ethanol in the presence of 0.05 M MgCl2 to remove contaminated soluble alginate.
It was shown by cellulose acetate membrane electrophoresis that the fucose-containing poly-saccharide thus obtained was free from water-soluble alginate, whereas the precipitate formed during the ethanol fractionation was mainly composed of water-soluble alginate.
By use of the procedure, the water-soluble alginate was found to be successfully removed from the algal polysaccharide.