タツナミガイのセルニーゼ

抄録

An extract from the acetone-treated powder of mid-gut gland of Dolabella auricularia, a marine millusk, was salted out with ammonium sulfate, then fractionated by successive gel filtrations on Sephadex G-50 and G-100 columns. A cellulase fraction practically free of β-glucosidase activity was obtained.
The cellulase preparation showed a typical endo-type hydrolysis mode for CMC. Its activity was optimal at pH 5.5 and the molecular weight was estimated to be 31, 000 by the gel filtration method. The elution patterns on isoelectric focusing of partially purified preparations suggested the presence of multiple forms in the Dolabella cellulase and β-glucosidase.