Techniques for manometric determination of the rate of tissue respiration were examined with various tissues of carp, focussing on substrate solution, oxygen pressure of gas phase, and method of preparing tissue. Physiological saline was suffcient as the solution for a short period determination. Pyruvate, fumarate, glutamate, glucose, or calcium were not necessary to add to the solution for the manometry of 30min. Ordinary air (PO2=155mmHg) gave, compared with 3% oxygen (PO2=22mmHg), higher values for hepatopancreas and ordinary muscle, and lower values for blood, while roughly same values for testis and ovary. Homogenized and pestled tissues gave much lower values than scissored or intact ones for various tissues. Scissored preparation made by scissoring tissue in a chilled vessel about 400 times until it becomes pasty is considered to give the most reliable results for various tissues.
- Nippon Suisan Gakkaishi is an official journal of the Japanese Society of Fisheries Science written in Japanese only.
The society publishes an English journal, Fisheries Science, as well, which accepts submissions from non-members of the society over the world. Detailed information is available in http://jsfs.jp/en/journals/fisheries-science.