Abstract
The attraction-effective fractions obtained from the whole frond of brown algae Ishige okamurai were further fractionated to several subfractions and these subfractions were examined on the attraction activities for black abalone Haliotis discus. The subfractions of 40 and 50% ammonium sulfate saturation-proteins, acidic and basic amino acids, and neutral and phospho-lipids were high in these activities.
In this context, various chemicals of proteins, amino acids and lipids, and nitrogenous bases, were investigated for the activities. Of proteins tested, human albumin, and α- and β-globulins were ascertained to be active attractants: albumin was most effective. Of L-amino acids, all basic amino acids, some neutral and acidic amino acids, amides, and imino acids were also ascertained to be active attractants, particularly ornithine and hydroxyproline being most effective. On the other hand, L-amino acids proved to be effective attractants, as opposed to D-ones. Of fatty acids and lipids, tristearin was high in the activity and the majority of phospholipids were effective, especially phosphatidyl-inositol being highest in the activity. Of nitrogenous bases, all volatile methylamines and ethylamines tested and some non-volatile ones were ascertained to be active: monomethylamine and γ-aminobutyric acid were most effective attractants.
