NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Tetrodotoxin in the Horseshoe Crab Carcinoscorpius rotundicauda Inhabiting Thailand
Attaya KungsuwanYuji NagashimaTamao NoguchiYasuo ShidaSunee SuvapeepanPanthip SuwansakornkulKanehisa Hashimoto
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1987 Volume 53 Issue 2 Pages 261-266

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Abstract

Further attempts were made to identify the toxins of the horseshoe crab Carcinoscorpius rotundicauda which causes sporadically food poisonings in Thailand.
Toxic C. rotundicauda eggs were crushed and extracted with water repeatedly. After being defatted with dichloromethane, the combined extracts were ultrafiltered to cut off more than 1, 000 dalton-substances. Toxic fractions were obtained from the filtrate by column chromatography on Bio-Gel P-2 and Bio-Rex 70.
Cellulose acetate strip electrophoresis, TLC, HPLC, and other instrumental analyses demon-strated that the horseshoe crab toxin was essentially composed of tetrodotoxin and related sub-stances, along with paralytic shellfish poisons as minor components.

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© The Japanese Society of Fisheries Science
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