ハマチ稚魚に対する酸化油脂の毒性とグルタチオンによる解毒効果

抄録

A 4-week feeding study and succeeding hematological analyses were carried out using fingerling yellowtail in order to test the interaction of dietary oxidized oil and glutathione. Major ingredients of the experimental diets were raw sand lance (61%) and raw mysis (40%). Supplemental level of oxidized cod viscera oil (POV, 84meq/kg) was 5% with or without glutathione supplementation (0.1%, reduced form). Fish fed the diet with the oxidized oil alone showed poor growth rate and high mortality (23% in 4 weeks). Although no sekoke disease like symptom was detected even in this dietary group, the hematological analyses revealed that feeding the oxidized oil lowered hematocrit level and serum neutral fat content, but increased glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities, and immunoglobulin M level in the serum as well as free amino acid levels. Supplementation of glutathione to the diet prevented these symptoms almost completely.