1988 年 54 巻 10 号 p. 1799-1802
Attempts were made to detect tetrodotoxin (TTX)-producing bacteria in the Thai horseshoe crab Carcinoscorpius rotundicauda, one of TTX-containing marine invertebrates. Thirteen dom-inant bacteria were isolated from the intestinal contents of live horseshoe crab specimens, and were cultured in a 2 × ORI medium at 20°C for 24h. Bacterial cells of each strain were harvested, ultrasonicated and ultrafiltered, and TTX and anh-TTX fractions were separated from the filtrate by Bio-Gel P-2 column chromatography. HPLC, UV and GC-MS analyses showed that all out of the 13 strains isolated produced anh-TTX, and one strain produced TTX and anh-TTX. The strain which produced both toxins was identified as Vibrio alginolyticus on the basis of biochemical and other characters.