Myosins were prepared from the ordinary muscle of 13 fish species such as skipjack, tuna, mackerel, and electrophoresed on SDS-polyacrylamide gradient gels. Resulting light chain regions were transferred to a GVHP membrane, and examined by immunoblotting technique for crossreactivity with the following anti-myosin light chain rabbit antisera: anti-skipjack A1, A2 and DTNB light chains, anti-round herring A1 and anti-Alaska pollack A1 antisera.
Different blotting patterns were obtained depending on antiserum species used. A1 and DTNB light chains generally showed a strong cross-reactivity, while A2 light chain did a rather weak reactivity.
It was suggested from these results that myosin light chains have primary to higher-order structures in common throughout the three types of light chain and the fish species examined.
- Nippon Suisan Gakkaishi is an official journal of the Japanese Society of Fisheries Science written in Japanese only.
The society publishes an English journal, Fisheries Science, as well, which accepts submissions from non-members of the society over the world. Detailed information is available in http://jsfs.jp/en/journals/fisheries-science.