Estimating Rate of Gene-Centromere Recombination at Eleven Isozyme Loci in the Salvelinus Species

Abstract

The present paper describes the successful induction of gynogenetic diploid and triploid animals in the brook trout Salvelinus fontinalis and the hybrid between the brook trout and the Japanese charr S. leucomaenis by application of hydrostatic pressure shock to eggs after fertilization with genetically inert sperm or normal sperm. Analyses on isozyme genotypic compositions in the parents and their gynogentetic and/or triploid offsprings made it possible to estimate the rates of gene-centromere recombination (x, 0≤x≤0.5) at eleven loci, indicating 0.341 at Alcohol dehydrogenase, 0.457 at α-Glycerophosphate dehydrogenase-A, 0.398 at Isocitrate dehydrogenase-C, 0.049 at Lactate dehydrogenase-A₁, 0.023 at Lactate dehydrogenase-A₂, 0.011 at Lactate dehydrogenase-B₂, 0.439 at Malate dehydrogenase-C/D, 0.465 at Malic enzyme-A, 0.443 at Phosphoglucose isomerase-A, 0.045 at Phosphoglucomutase-B and 0.489 at Superoxide dismutase. Map distance thus obtained in the present work revealed the value close to that reported in the other species of the genus Salvelinus and Salmo at each locus of probable homology.