NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
Introduction of Sperm of Common Carp Cyprinus carpio into Eggs of Ginbuna Carassius langsdorfii by Heat Shock Treatment and Its Confirmation by DNA Markers
Shi DongKenichi OharaNobuhiko Taniguchi
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Keywords: RAPD-PCR
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1997 Volume 63 Issue 2 Pages 201-206

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Abstract

The eggs of a Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperms of a common carp Cyprinus carpio. A part of the eggs was treated by heat shock: 40°C for 1.0min at 5min after fertilization.
The average of erythrocytic major diameter of offsprings from non-treated eggs was 17.4μm (17.1-17.7μm). The patterns of glucosephosphate isomerase (GPI), RAPD-PCR (random primer: OPA-04, 09, 15), and DNA fingerprinting (restriction endonuclease: Hae III; probe: YNZ 22) in the offsprings were identical with maternal fish.
The average of erythrocytic major diameter of offsprings from treated eggs was 20.6μm (20.3-20.9μm). The patterns of GPI, RAPD-PCR, and DNA fingerprinting in the offsprings were composed of all bands of maternal fish and partial bands of paternal fish.
Therefore, the results obtained in this study suggest that the offsprings from non-treated eggs were triploid clones, and the offsprings from treated eggs might be tetraploid composed of genome from both parents.

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© The Japanese Society of Fisheries Science
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