コイ肝膵臓における代謝中間体の含量およびphosphofructokinase活性への関与

示野 貞夫; 四方 崇文; 岩永 俊介; 杉田 毅

doi:10.2331/suisan.64.110

示野貞夫, 四方崇文, 岩永俊介, 杉田毅

著者情報

キーワード: コイ, 解糖中間体, ヌクレオチド, 解糖, 解糖調節

ジャーナルフリー

64 巻 (1998) 1 号 p. 110-115

DOI https://doi.org/10.2331/suisan.64.110

詳細

公開日
公開日: 2008/02/29 [早期公開] 公開日: - 受付日: 1997/03/11 改訂日: - 受理日: -
訂正情報
訂正日: 2008/02/29 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : 4) T. Shikata, S. Iwanaga, and S. Shimeno: Metabolic response to acclimation temperature in carp. Fisheries Sci., 61, 512-516 (1995).
5) S. Shimeno, D. Kheyyali, and T. Shikata: Metabolic response to dietary lipid to protein ratios in common carp. Fisheries Sci., 61, 977-980 (1995).
10) W. Lamprecht and I. Trautschold: Adenosine-5′-triphosphate. Determination with hexokinase and glucose-6-phosphate dehydrogenase. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 4, Academic Press, New York, 1974, pp. 2101-2110.
11) D. Jaworek, W. Gruber, and H. U. Bergmeyer: Adenosing-5′-diphosphate and adenosine-5′-monophosphate. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 4, Academic Press, New York, 1974, pp. 2127-2131.
12) G. Lang and G. Michal: D-glucose-6-phospate and D-fructose-6-phosphate. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 3, Academic Press, New York, 1974, pp. 1238-1242.
13) S. Dagley: Citrate. UV spectrophotometric determination. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 3, Academic Press, New York, 1974, pp. 1562-1565.
14) H. U. Bergmeyer and G. Michal: D-Glucose-1-phosphate. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 3, Academic Press, New York, 1974, pp. 1233-1237.
15) T. H. Massey and W. C. Deal, Jr.: Unusual, metabolite-dependent solubility properties of phosphofructokinase. The basis for a new and rapid purification from liver, kidney, and other tissues. J. Biol. Chem., 248, 56-62 (1973).
16) J. Gras, D. Gautheron et J. Henry: Fractions et phosphorylees et nucleotides libres de divers organes de la Truite Arc-en-Ciel. C. R. Acad. Sc. Paris, 265, 1547-1549 (1967).
17) H. Lin, D. R. Romsos, P. I. Tack, and G. A. Leveille: Effect of fasting and feeding various diets on hepatic lipogenic enzymes in coho salmon. J. Nutr., 107, 1477-1483 (1977).
19) K. Nagashima, T. Nakagawa, and F. Nagayama: Properties of phosphofructokinase from carp, eel, and rainbow trout liver. Nippon Suisan Gakkaishi, 55, 897-903 (1989).
20) J. E. Halver: Nutrition of salmonoid fishes III. Water soluble vitamin requirements of chinook salmon. J. Nutr., 62, 225-243 (1957).
22) M. J. Walton and C. B. Cowey: Aspects of intermediary metabolism in salmonid fish. Comp. Biochem. Physiol., 73B, 59-79 (1982).
23) T. W. Moon and G. D. Foster: Tissue carbohydrate metabolism, gluconeogenesis, and hormonal and environmental influences. in “Biochemistry and Molecular Biology of Fishes” (ed. by P. W. Hochachka and T. P. Mommsen), Vol. 4, Elsevier, Amsterdam, 1995, pp. 65-100.

Right : 4) T. Shikata, S. Iwanaga, and S. Shimeno: Metabolic response to acclimation temperature in carp. Fisheries Sci., 61, 512-516 (1995).
5) S. Shimeno, D. Kheyyali, and T. Shikata: Metabolic response to dietary lipid to protein ratios in common carp. Fisheries Sci., 61, 977-980 (1995).
10) W. Lamprecht and I. Trautschold: Adenosine-5'-triphosphate. Determination with hexokinase and glucose-6-phosphate dehydrogenase. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 4, Academic Press, New York, 1974, pp. 2101-2110.
11) D. Jaworek, W. Gruber, and H. U. Bergmeyer: Adenosing-5'-diphosphate and adenosine-5'-monophosphate. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 4, Academic Press, New York, 1974, pp. 2127-2131.
12) G. Lang and G. Michal: D-glucose-6-phospate and D-fructose-6-phosphate. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 3, Academic Press, New York, 1974, pp. 1238-1242.
13) S. Dagley: Citrate. UV spectrophotometric determination. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 3, Academic Press, New York, 1974, pp. 1562-1565.
14) H. U. Bergmeyer and G. Michal: D-Glucose-1-phosphate. in “Method of Enzyme Analysis” (ed. by H. U. Bergmeyer), Vol. 3, Academic Press, New York, 1974, pp. 1233-1237.
15) T. H. Massey and W. C. Deal, Jr.: Unusual, metabolite-dependent solubility properties of phosphofructokinase. The basis for a new and rapid purification from liver, kidney, and other tissues. J. Biol. Chem., 248, 56-62 (1973).
16) J. Gras, D. Gautheron et J. Henry: Fractions et phosphorylees et nucleotides libres de divers organes de la Truite Arc-en-Ciel. C. R. Acad. Sc. Paris, 265, 1547-1549 (1967).
17) H. Lin, D. R. Romsos, P. I. Tack, and G. A. Leveille: Effect of fasting and feeding various diets on hepatic lipogenic enzymes in coho salmon. J. Nutr., 107, 1477-1483 (1977).
19) K. Nagashima, T. Nakagawa, and F. Nagayama: Properties of phosphofructokinase from carp, eel, and rainbow trout liver. Nippon Suisan Gakkaishi, 55, 897-903 (1989).
20) J. E. Halver: Nutrition of salmonoid fishes III. Water soluble vitamin requirements of chinook salmon. J. Nutr., 62, 225-243 (1957).
22) M. J. Walton and C. B. Cowey: Aspects of intermediary metabolism in salmonid fish. Comp. Biochem. Physiol., 73B, 59-79 (1982).
23) T. W. Moon and G. D. Foster: Tissue carbohydrate metabolism, gluconeogenesis, and hormonal and environmental influences. in “Biochemistry and Molecular Biology of Fishes” (ed. by P. W. Hochachka and T. P. Mommsen), Vol. 4, Elsevier, Amsterdam, 1995, pp. 65-100.

訂正日: 2008/02/29 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

The metabolic intermediate concentrations and enzyme activities were determined in the hepatopancreas of carp Cyprinus carpio fed for 30 days with diets high in protein, lipid, and carbohy-drate. The effect of physiological concentrations of the intermediates on the activity of purified phos-phofructokinase (PFK) was also investigated. The dietary treatments induced slow but marked changes in activities of hepatopancreatic enzymes. This suggested an enzyme induction in the fish hepatopancreas but could not account for the rapid response of body composition to the dietary treat-ments. Each concentration of ATP, ADP, AMP, G1P, G6P, F6P, and FDP in the hepatopancreas ranged from 10^-3M to 10^-5M, corresponding to the Km values for many glycolytic enzymes such as PFK. These physiological concentration ranges of ATP, AMP, F6P, and citrate markedly and reasonably affected in vitro PFK activity. The results suggest that in vivo glycolytic activity can be regulated not only by enzyme induction but also by PFK activity without enzyme induction through changes in the physiological concentrations of metabolic intermediates as substrate and effector.

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