Abstract
A system was developed for exposing cell cultures to the gaseous photochemical reaction products. The gaseous photochemical reaction products was produced by photochemical reaction of a hydrocarbon-NO2-air system using the evacuable photochemical smog chamber. Quantitative analysis of gaseous compounds in the chamber air was done in situ by a long-path Fourier transform infrared absorption spectroscopy. In order to expose cell cultures to the gaseous photochemical reaction products, a system was designed and constructed in which monolayers cultured in culture square bottles are being rotated by an apparatus of roller bottle culture. Cell cultures of Chinese hamster V 79 cells were exposed to the photochemical reaction products of propylene plus NO2 (initial concentrations of propylene: NO2; 0.5 ppm: 0.25 ppm, 1.0 ppm: 0.5 ppm, 2.0 ppm: 1.0 ppm, and 3.0 ppm: 1.5 ppm) for 2 hr and toxicity was measured as inhibition of cell growth 24 hr after exposure. Growth was inhibited at all concentrations used in this study.
The system described was found to be effective for investigating the gaseous photochemical reaction products for cultured cells.
