2013 年 35 巻 p. 24-34
A method to observe animal internal tissues of alcohol specimens is proposed using resin impregnation and geological grinding technique. Animal specimens were embedded in Spurr resin and ground to expose the internal organs. In this method, both hard and soft tissues can be simultaneously observed by using a stereo-microscope. Thin sections of the specimens were also prepared for transmitted-light microscopy. This method can retain the texture and color of the tissue. It was applied to an ophiuroid with calcite skeleton, a sponge with silica skeleton, and a beetle with hard cuticle exoskeleton.