Collembola, also called springtails, are wingless microarthropods that are widespread in terrestrial habitats and mainly inhabit soil. Applications of collembolan DNA barcoding are expected in taxonomy, ecology, and environmental sciences to unveil cryptic diversity, to provide a rapid and easy method for community measurements, and to provide an accurate identification method for ecotoxicological test species for non-taxonomists. Collembolan DNA barcoding was initiated in 2004 with Canadian specimens and is now ongoing in several countries. A rapid method for non-destructive DNA extraction from individual specimens has been developed to preserve voucher specimens. A quantitative protocol for Collembola DNA metabarcoding has also been developed. Collembolan DNA barcode data are gradually accumulating, although data on type specimens and topotypes are still scarce. Because several cryptic lineages have been found through DNA barcoding, taxonomists who can classify cryptic lineages and describe cryptic species are needed.