The Bulletin of Tokyo Dental College
Print ISSN : 0040-8891
Short Communication
Depolarization-induced Intracellular Free Calcium Concentration Increases Show No Desensitizing Effect in Rat Odontoblasts
Yuki KojimaAsuka HigashikawaMaki KimuraMasaki SatoHiroyuki MochizukiKazuhiro OguraToshiyuki SaseAkinori ShinyaKunio KobuneTadashi FuruyaToru SatoYoshiyuki ShibukawaMasakazu Tazaki
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2015 Volume 56 Issue 2 Pages 131-134

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Abstract

Odontoblasts play an important role in the transduction of the sensory signals underlying dentinal pain. Transmembrane voltage-independent Ca2+ influx in odontoblasts has been well described. Voltage-dependent Ca2+ influx has also been reported, but its biophysical properties remain unclear. The aim of the present study was to investigate the desensitizing effect of voltage-dependent Ca2+ influx in rat odontoblasts by measuring depolarization-induced intracellular free Ca2+ concentrations ([Ca2+ ]i ). Odontoblasts on dental pulp slices from newborn rats were acutely isolated and [Ca2+ ]i measured by using fura-2 fluorescence. Repeated application of extracellular high-K+ solution (50 mM), which induces membrane depolarization-elicited repeated and transient increases in [Ca2+ ]i in the presence of extracellular Ca2+. Increases in depolarization-induced [Ca2+ ]i showed no significant desensitizing effect (p >0.05; Friedman test). These results suggest that odontoblasts express a voltage-dependent Ca2+ influx pathway with no desensitizing properties.

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© 2015 by Tokyo Dental College, Japan
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