11 ^<13>C NMRのGibberellinの構造解析並びに生化学的研究への応用-(1)

抄録

The recent advances of the instruments and techniques made it possible to apply the ^<13>C NMR to the naturally occurring substances. In our attempts to use the method not only for the structural determination but also for the biosynthetic study of gibberellins, we analysed ^<13>C NMR spectra of several C_<19> gibberellins or their methyl esters. Spectra were obtained by using PFT technique with JNM PS 100 in deuteroacetone and/or deuteropyridine solution with TMS as an internal standard. The assignment was performed by analysing the proton off-resonance decoupled spectra and by compairing the spectra of gibberellin homologues which have similar structures but differ in substitution patterns. And ^<13>C NMR was applied to the structural determination of gibberellin A_<40>(GA_<40>). The comparison of the ^<13>C NMR spectrum of GA_<40>-Me with those of GA_4-Me and GA_9-Me, suggested that GA_<40> is an isomer of GA_4, and has a hydroxyl group on 2-C. The configuration of the hydroxyl was decided to be α (axial) from its PMR spectrum.