天然有機化合物討論会講演要旨集
Online ISSN : 2433-1856
セッションID: 36
会議情報
36 前胸腺によるエクダイソンの生合成
池川 信夫粟田 則男茅野 春雄桜井 勝大滝 哲也宮崎 浩石橋 正兀阿武木 秀夫
著者情報
会議録・要旨集 フリー

詳細
抄録

There has been no direct evidence to establish that the prothoracic glands are responsible for ecdysone synthesis. Our improved culture system, in which the isolated prothoracic glands of the Bombyx silkworm were cultivated in hemolymph medium under a continuous supply of excess oxygen, led to first successful result. A typical set of data (Table 1) reveals that a considerable amount of hormonal activity is found in the medium. The hormonal fraction was extracted from the culture medium and applied to a TLC plate. The results revealed that all the hormonal activity was located in a distinct region corresponding to α or β-ecdysone. The ecdysone fraction was submitted to high pressure liquid chromatography. Only fraction 8 (Fig. 5) having the same retention time with α-ecdysone had the hormonal activity On the other hand, a specific gas phase analysis of zooecdysones using mass fragmentography of trimethylsilyl derivative was developed. Quantitative estimation of 10^<-10> g level of α and β-ecdysone can be obtained by focusing at m/e 564 and 561, respectively. Analysis of the fraction 8 by this technique clarified that the hormone obtained from organ culture is α-ecdysone.

著者関連情報
© 1974 天然有機化合物討論会電子化委員会
前の記事 次の記事
feedback
Top