Trends in Glycoscience and Glycotechnology
Online ISSN : 1883-2113
Print ISSN : 0915-7352
MINIREVIEW (Japanese)
Photocrosslinking Sugars for Capturing Glycan-dependent Interactions (Jpn. Ed.)
Akiko FujitaJennifer J. Kohler
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JOURNALS FREE ACCESS

2015 Volume 27 Issue 156 Pages J1-J7

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Abstract

Photocrosslinking approaches are an important strategy to covalently capture biomolecular interactions. Covalent capture by photocrosslinking is particularly powerful for glycan-dependent interactions because these binding events are typically low-affinity and unable to withstand common purification protocols. Implementation of photocrosslinking methods is based on the incorporation of a photoactivatable crosslinking functional group onto glycan-containing molecules. Upon activation with UV radiation, the photocrosslinking group is converted to a reactive species, which forms a covalent bond with neighboring molecules. Resulting crosslinked complexes can be analyzed by immunological and mass spectrometry techniques to identify glycan-dependent binding partners. In some cases, photocrosslinker incorporation can be achieved in living cells, enabling detection of glycan-dependent interactions in their native context. In this mini-review, we describe new sugar-based photocrosslinking reagents and illustrate how they have been used to photochemically characterize glycan-dependent interactions.

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© 2015 FCCA(Forum: Carbohydrates Coming of Age)
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