Trends in Glycoscience and Glycotechnology
Online ISSN : 1883-2113
Print ISSN : 0915-7352
ISSN-L : 0915-7352
Article for JSCR 40th Anniversary Issue
Towards a Novel Platform for Mucin Analysis
Akihiko Kameyama
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2019 Volume 31 Issue 181 Pages SE40-SE41

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Abstract

Characterization of mucins are a difficult task because of their large molecular sizes, highly heterogeneity of glycosylation, and resistance to proteases. For the discovery of mucin biomarkers, supported molecular matrix electrophoresis (SMME), a relatively easy technique for mucin separation, has been developed. Using SMME, mucins and their O-linked glycans from pancreatic juice, cultured cells, and salivary glands were characterized. Furthermore, chemical inertness of the SMME membranes was effectively utilized to develop a novel staining method and glycan stripping method for mucins located on the membrane.

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© 2019 FCCA (Forum: Carbohydrates Coming of Age)
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