A Comparison of the Colorimetric Determinations of Epinephrine by Folin, Cannon and Denis, and by Suto and Inouye with the Rabbit Intestine Segment Method
1928 年 10 巻 6 号 p. 528-545
詳細
1928 年 10 巻 6 号 p. 528-545
1. The extract of the bovine suprarenal medulla prepared by Folin Cannon and Denis' procedure was determined for epinephrine colorimetrically by Folin, Cannon and Denis and by means of the rabbit intestine segment method in parallel. The values yielded by both methods did not coincide with each other, the ratio of the colorimetrical to the biological was 1:1.1 to 1:2.1 in five experiments.
2. Of the extract of medullary tissue of bovine suprarenal capsule, made according to Suto and Inouye, the parallel estimations with the colorimetrical method of Suto and Inouye and the rabbit intestine strip test were conducted. These values also did not check with each other, and in fact the latter yielded invariably a somewhat higher value, 1:1.3 to 1:2.3 being calculated as the ratio.
3. Suto's extract cannot be simply applied to the rabbit intestine strip. The precautions taken against interfering effects are noted in the text.
