Glycosaminoglycans Isolated from Rabbit Femur

Abstract

Complex carbohydrate fraction (E-Fr) was extracted with 40% aqueous EDTA (pH 7.4) from the acetone-dried bone powders of rabbit femur. E-Fr was separated into five fractions, CPC-S Fr, 0.3 M Fr, 0.5 M Fr, 0.75 M Fr and 1.0 M Fr by the successive chromatography on a CM-Sephadex C-25 column and a CPC (cetylpyri-dinium chloride)-cellulose column. Subsequently, CPC-S Fr was purified by the chromatography on a DEAE-Sephadex A-50 column. The resulting major fraction (CPC-S-M) was identified as a sulfated glycoprotein-like substance carrying a small amount of chondroitin sulfate A. On the other hand, 0.75 M Fr and 1.0 M Fr were purified separately by the rechromatography on a CPC-cellulose column. The purified preparations of 0.75 M Fr and 1.0 M Fr were identified as a glycoprotein-bound chondroitin sulfate A and protein-free chondroitin sulfate A, respectively.